Question

Describe the necessary steps from observing a specimen with 4X objective lens to observing the same specimen with 100X objective lens under the light microscope.

Answer 1

In order to observe a specimen under 4X objective lens, the following procedures must be observed:

• Set up the microscope ensuring that the lens have been adequately cleaned and the light source is working
• Mount the object on a slide and place the slide on the stage of a clean microscope.
• Switch the revolving nose piece to ensure that the 4X objective is in position
• Switch on the light source
• Bring the object into focus by using coarse and fine adjustment knobs.
• Ensure that the image of the object is centered on the stage.

In order to observe the same specimen under the 100X objective:

• Switch the revolving nose piece to fix the 100x objective in place.
• Only use the fine adjustment knob to bring the image into focus
• If the image will not resolve properly, a few drops of immersion oil may be applied to the slide to improve resolution.

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