Answer:
The first attached figure below shows the design of an agarose gel with four sequencing reactions. The second figure presents a photo of an agarose gel, so that you can better understand how the bands are represented in this gel.
Explanation:
To view the bands of four sequencing reactions on an agarose gel, you will need to use a melted agarose gel, plastic combs suitable for that reaction and a container suitable for that type of gel. You will place the plastic combs in the container and pour all the gel into the vat and wait for the melted gel to solidify. The plastic combs will form holes in the hardened gel where the DNA samples will be placed.
Once the gel is hardened, you will remove the plastic combs and begin to apply the sequenced DNA.
The sequenced DNA samples will be mixed with a dye, usually bromophenol blue, which will allow you to visualize the bands formed on the gel. You will also apply the dye to a sample without DNA containing only the dye, which serves as a comparison for the size of the bands.
Each sample of DNA will be plated in the column of holes formed by the plastic combs. Then, this container, with the gel, will be placed in a larger container that contains a loading buffer. The larger container will be closed and an electric field will be applied that will force the DNA samples to be moved from one pole to another inside the container, in this case, the samples leave the negative pole for the positive pole.
After a few minutes, it is possible to visualize the DNA displacement and at the end of the procedure it will be possible to visualize the formation of bands as shown in the drawing and in the figure below. The size of these bands can be compared and analyzed.
Answer:
The conversion of ADP to ATP using light energy
Explanation:
Photophosphorylation is a stage in photosynthesis in which ADP is converted to ATP using sunlight energy.
Note that, in photophosphorylation, 2 ATPs are produced
This process is fundamental in maintaining a stable energy level in our eco-system.
Answer:
Creative Biogene CRISPR/Cas9 Platform provides comprehensive gene editing services for various cell lines, especially for primary T cells. With years of experience, our talented scientists have established an efficient assay to edit genome in T cells by CRISPR/Cas9. At CRISPR/Cas9 PlatformCB, we offer a gene knockout service for customer as well as isolation of primary T cell service. Based on our excellent platform, our staff are focusing on supplying the best services and products.
Answer:
1. Define a Question to Investigate
As scientists conduct their research, they make observations and collect data. The observations and data often lead them to ask why something is the way it is. Scientists pursue answers to these questions in order to continue with their research. Once scientists have a good question to investigate, they begin to think of ways to answer it.
2. Make Predictions
Based on their research and observations, scientists will often come up with a hypothesis. A hypothesis is a possible answer to a question. It is based on: their own observations, existing theories, and information they gather from other sources. Scientists use their hypothesis to make a prediction, a testable statement that describes what they think the outcome of an investigation will be.
3. Gather Data
Evidence is needed to test the prediction. There are several strategies for collecting evidence, or data. Scientists can gather their data by observing the natural world, performing an experiment in a laboratory, or by running a model. Scientists decide what strategy to use, often combining strategies. Then they plan a procedure and gather their data. They make sure the procedure can be repeated, so that other scientists can evaluate their findings.
4. Analyze the Data
Scientists organize their data in tables, graphs, or diagrams. If possible, they include relevant data from other sources. They look for patterns that show connections between important variables in the hypothesis they are testing.
5. Draw Conclusions
Based on whether or not their prediction came true, scientists can then decide whether the evidence clearly supports or does not support the hypothesis. If the results are not clear, they must rethink their procedure. If the results are clear, scientists write up their fi ndings and results to share with others. The conclusions they draw usually lead to new questions to pursue.
