Where does the light independent reaction take place

Biology

1 answer:

hjlf2 years ago

4 0

Answer:

stroma

Explanation:

The light-independent reactions represent the known Calvin–Benson–Bassham (CBB) cycle which takes place in the stroma of chloroplasts and is the primary pathway of carbon fixation of C3 plants [119].

You might be interested in

If i have stress fracture of my radius bone what part of my body i have broken

laila [671]

Forearm.
____________

4 0

3 years ago

Since beans contain the essential amino acid methionine, beans are considered complete protein sources.

Leni [432]

The above statement is false.
A complete protein is a source of protein that contains an adequate proportion of all nine essential amino acids necessary for the dietary needs of humans.
For example, meat, seafood, eggs and dairy are all complete proteins, while plant foods like beans, whole-grains and brown rice are incomplete proteins.

6 0

3 years ago

Read 2 more answers

A DNA fragment is introduced into the lacZ gene of a plasmid, which also contains an ampicillin resistance gene. What is the app

zhenek [66]

Answer: The bacteria transformed with this particular plasmid will form white colonies on the plates containing ampicillin and Xgal.

Explanation: The lacZ gene produces an enzyme called β-galactosidase which is responsible for the breakdown of lactose into glucose and galactose. The lacZ gene is one of the three genes (the other two being lacA and lacY) of the lac operon which is responsible for the transport and mechanism of lactose in E. coli and many other bacteria.

In recombinant DNA technology, when a plasmid is to be used to transform a host cell, such markers are used to help screen the transformed cells from the ones that have not taken up the plasmid. Xgal present in the plates is an artificial substrate which is hydrolyzed by

β-galactosidase into 5-bromo-4-chloro-indoxyl which will dimerize and oxidise into 5,5'-dibromo-4,4'dichloro-indigo. This is a blue pigment which will give blue color to the bacterial cells. Introducing a DNA fragment in this lacZ gene will make it non-functional so it will not be able to produce the enzyme.

Therefore, when a bacterial cell is transformed with a plasmid containing ampicillin resistance gene and a DNA fragment introduced in the lacZ gene and then grown on plates containing ampicillin and Xgal, white colored colonies will appear. The white colonies will show the bacterial cells that have successfully taken up the plasmid with the DNA fragment incorporated in the lacZ gene as this will render the gene non-functional and will not produce β-galactosidase which will breakdown Xgal to give blue colonies. Since the plates contain ampicillin, only the bacterial cells that have been successfully transformed with the plasmid ( the ones that have the DNA fragment and the ones without it) will grow as the ampicillin resistance will give them resistance against ampicillin in the plates. The bacterial cells that have not taken up the plasmid will not be resistant to ampicillin and will not form colonies on the plate.

This is called blue-white screening which is used to identify successfully transformed host cells. A picture of this is given in the attachment, taken from the following website:

https://www.mun.ca/biology/scarr/Blue_&_White_Colonies.html