1. Radiation - energy transmits through particles that ionize. Conduction - heat is transferred through a substance without moving the material. Convection - transfer of heat through movement.
2. Earth receives energy from the sun, which is transferred between Earth and its atmosphere.
3.<span>Wildland fires, dust storms, and volcanic activity. They release CO2, CH4, N20, and sulfur dioxide.</span>
4. Burning coal, releases carbon dioxide and other pollutants. Gasoline, releases air pollution. Factories, releases carbon dioxide, methane, and nitrous oxide.
Answer:
Cas9-sgRNA cannot align with targeted DNA sequence
Explanation:
Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.
The technology that was used to produce the results illustrated in the image is gel electrophoresis and it is used for separating deoxyribonucleic acid (DNA) fragments.
Gel electrophoresis can be defined as a technology that is used in the laboratory for the separation of mixtures of deoxyribonucleic acid (DNA), ribonucleic acid (RNA) or other macromolecules such as proteins based on molecular size and charge.
In gel electrophoresis, an electric current is applied to the deoxyribonucleic acid (DNA) samples so as to create fragments that can be used to compare the various samples of deoxyribonucleic acid (DNA).
The steps involved in gel electrophoresis include the following:
- Extraction of deoxyribonucleic acid (DNA).
- DNA isolation and amplification.
- Addition of deoxyribonucleic acid (DNA) to the gel wells.
- Application of an electric current to the gel.
- The separation of DNA bands based on molecular size and charge.
- Deoxyribonucleic acid (DNA) bands are stained.
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Oil sticks to birds preventing them from flying. Whales and dolphins and other creatures that use blow holes can get oil into their respiratory system, making it difficult to breathe. Animals can also ingest the oil. This can be fatal and/or deadly in the short or long term.