Step 1: Isolate the two kinds of DNA.
Step 2: Treat the plasmid and foreign DNA with the same restriction enzyme.
Step 3: Mix the foreign DNA with chopped plasmids.
Step 4: Add DNA ligase.
Organelles exist within cells, which exist within tissues. Communities exist within ecosystems, which exist in the biosphere.
Answer:
I think d tell me if im wrong
Explanation:
Answer: Because the cell will expand and burst
When a cell is frozen, that means the cell temperature is going to be lowered extremely. Ice has more volume than water which is why it floating inside water. That's why if you put water in a plastic container then freeze it, the container will get pressured and swollen. If the container is not elastic enough(metal, glass), it will burst.
Same like that, the cell that got frozen can be burst too.