The restriction endonuclease EcoRI cleaves the DNA sequence 5'-GAATTC-3' in such a way as to leave a 5' overhang, which is often referred to as a "sticky end". To 5' overhang with the complementary sequence this sticky end adhere.
- The palindrome EcoRI restriction site breaks DNA after G and creates sticky ends with AATT. G/corresponding AATTC's DNA sequence is CTTAA/G, where "/" designates the location of the peptide bond break.
- When the ssDNA sequence 5'-GAATTC'-3 is recognised by the restriction endonuclease EcoRI, a single-strand cut is made between the G and A nucleotides.
- At the ultimate end of double-stranded DNA, an overhang is single-stranded DNA. This can occur on either strand, and if it does, there will either be a 5' overhang on the 5' end of the strand or a 3' overhang on the 3' end of the strand.
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The circuit has to be closed, so B.
If the circuit was open, the flow of energy wouldn't be able to reach the light bulb.
This is most likely to occur during the Anaphase
The Galapagos Islands would be found in the Pacific Ocean. From the nearest point in the United States, they are about 2,636 miles away.
