Question

A biologist wishes to take a useful gene found in pine trees and introduce it into some bacterial cells. Her experimental procedure is given below.
1. Use a restriction enzyme to cut a gene out of pine tree DNA.
2.Use a restriction enzyme to cut open a plasmid.
3.Treat the plasmid with DNA polymerase.
4.Introduce the plasmid into bacterial cells.
Which step shows an error in the biologist's procedure?

Answer 1

Answer:

3.Treat the plasmid with DNA polymerase.

Explanation:

DNA polymerase will not be useful in this step of the experiment.

In the third step of the experiment shown in the question above, it would be correct to use the enzyme called ligase, so that the plasmid was linked to the pine gene.

DNA polymerase cannot make this binding since the function of this enzyme is to allow DNA replication. As the polymerase was used in the third step of the experiment, the scientist will not succeed when trying to introduce the plasmid into bacterial cells, since this plasmid does not have the pine gene.

Answer 2

3 rd step is wrong as we have to combine it with the pine tree gene and for that we have to use ligase !! DNA polymerase will not help in making recombinant DNA !!

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