The restriction endonuclease EcoRI cleaves the DNA sequence 5'-GAATTC-3' in such a way as to leave a 5' overhang, which is often referred to as a "sticky end". To 5' overhang with the complementary sequence this sticky end adhere.
- The palindrome EcoRI restriction site breaks DNA after G and creates sticky ends with AATT. G/corresponding AATTC's DNA sequence is CTTAA/G, where "/" designates the location of the peptide bond break.
- When the ssDNA sequence 5'-GAATTC'-3 is recognised by the restriction endonuclease EcoRI, a single-strand cut is made between the G and A nucleotides.
- At the ultimate end of double-stranded DNA, an overhang is single-stranded DNA. This can occur on either strand, and if it does, there will either be a 5' overhang on the 5' end of the strand or a 3' overhang on the 3' end of the strand.
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<em><u>The water cycle describes how water evaporates from the surface of the earth, rises into the atmosphere, cools and condenses into rain or snow in clouds, and falls again to the surface as precipitation. ... The cycling of water in and out of the atmosphere is a significant aspect of the weather patterns on Earth.</u></em>
A. enough statistical support for the research hypothesis when there is not
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A trophic cascade is a side-effect when a trophic level (species) of the ecosystem is reduced or removed. Their ecological species interactions keep entire ecosystems balanced. Hence, suppressing their numbers or changing their behavior due to predation risk, results in an ecological imbalance or a trophic cascade.
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