Answer:
b. Forward or reverse primers
Explanation:
Sanger sequencing is a technique of DNA sequencing based on the extension of DNA fragments with variable sizes terminated with dideoxynucleotides at the 3′ end. This technique was developed by Frederick Sanger in 1977. In Sanger sequencing, a short primer is added in order to bind by complementarity to the target DNA region of interest. Subsequently, a DNA polymerase adds nucleotides (A, T, C and G) in the 5'-3' direction. Finally, the extension of the DNA strand is stopped by adding dideoxynucleotides, which are nucleotide analogs (i.e., modified nucleotides) that act as DNA synthesis terminators.
Answer:
during the Cambrian Period of the Paleozoic Era.
Explanation:
The Paleozoic is the earliest era (of three) of the Phanerozoic Eon and is composed of six geologic periods: the Cambrian, Ordovician, Silurian, Devonian, Carboniferous, and Permian (in that order from earliest to the latest). Fish first appeared about 530 million years ago during the Cambrian explosion.
Enzyme responsible for replacing RNA primers with DNA is a type of: DNA ligase. The enzyme responsible for proofreading a growing DNA strand and for replacing mismatched nucleotides is:helicase.
Answer and Explanation
Mendel selected a group of dwarf plants and sel pollinated them by dusting mature pollen grains onto them. He then collected the resulting seeds and planted them and noticed these seeds germinated and grew into dwarf plants only.
He also selected tall plants and self-pollinated them. the resulting seeds he observed that they grew into a micture of tall and dwarf plants. He took the seeds of tall plants only and repeated the experiments for many generations until he obtained only tall plants.
He then crossed cross-pollinated purebreeds tall garden pea with pure breed variety. He planted the seeds and observed the offsprings were all tall plants. He crossed two of these tall offsprinf from the F1 and planted the resulting seeds. the second filial generation consisted of a mixture of tall and dwarf plants.
He counted these plants and noted that the ratio of tall to dwarf was about 3:1. From this, he postulated that there are now laws governing inheritance.The law of independent assortment and law of segregation.
Answer:
May alter the specificity for its substrate
Explanation:
The active site of an enzyme refers to the specific region of an enzyme that serves as the binding site for its one or more substrates. Binding of substrates to the active site of their enzymes is required for catalysis. Enzymes are highly specific for their substrates. Type of amino acids present in the active site of the enzymes and their interactions with substrates regulate the specificity of the enzyme. If a mutation substitutes the amino acid of the active site, it may increase or decrease the specificity of the enzyme for its substrate.
