Engineering should be the answer because to create a great experiment, it has to be well put together.
<span>Homologous (This means they are the same chromosome from each parnet, matched together) </span>
<span>Negatively charged R-groups in the primary structure.
This is because nickel acts as a cation, therefore you would be attracting anions, in this case it would be on the proteins that would be binding to the column. Once they bind to the matrix a was buffer of high salts can elute the proteins.</span>