The enzyme Taq polymerase is required in the PCR process because it is the main enzyme that synthesizes the new DNA strands complementary to the template strand and without the activity of this enzyme the PCR will be useless and it cannot make the copy of the given strand in any way. Thus, this enzyme is very much necessary for the efficient working of the PCR.
Polymerase Chain Reaction is a very efficient technique that can able to detect and copy even a small amount of DNA by performing a set of reactions at different temperatures.
The PCR process begins with the denaturing of the template strand at 95°C, followed by annealing in which the appropriate primers bind to the single strands of DNA at 50-56°C, followed by extension at 72°C in which the thermostable Taq polymerase synthesizes the strand complementary to the template DNA.
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Answer:
Computer automation is used mostly by industry in factories. However, it is also useful in science. Machines can be used to run automated tests and complete simple tasks. One example of a machine used in science is the robotic arm that is used to handle radioactive samples.
Explanation:
I don't know what the expieraments about but I can say its rather B or C the independent variable is the variable that you are testing.
Explanation:
These questions are related to the extraction of DNA using detergents and ethanol.
1. Consistency and texture- the extracted DNA is white, spongy and moist or slime in nature.
2. Salt is used during the extraction process as the DNA is negatively charged molecule and to neutralize the negative charge which will allow the formation of precipitate.
3. The DNA is soluble in aqueous solution as the DNA is a negatively charged molecule therefore the negative charge makes it a polar molecule and it can form the interactions in the aqueous solution.
4. RNA could be present in the aqueous solution along with the DNA as both are the nucleic acid.
5. The base-pairing rule was suggested by the Chargaff who proposed that Adenine binds thymine and guanine binds cytosine.
5. The binding of adenine to thymine involves two hydrogen bonds and between guanine to cytosine involves three hydrogen bonds.
Answer: The effect will be either an abnormal protein, a non functional protein or a protein with altered function or functions.
Explanation: Every protein performs at least one specific function. Example is the case of sickle cell disease where a protein called haemoglobin which is responsible for oxygen transport in the body is mutated. In the sickle haemoglobin, valine replaces glutamate at position six of the two beta chains. Haemoglobin is a protein with four subunits made up of two alpha chains and two beta chains. The substitution of glutamate residue withwresults valine results in the haemoglobin having low affinity for oxygen binding and consequently, inefficient supply of oxygen to the cells.
