Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Cora might have been diagnosed with another disease that presents similarly as tardive dyskinesia (this disease is a common adverse effect of antipsychotic drugs). Her misdiagnosis is most likely Sydenham's Chorea which affects people with rheumatic fevers. Other differentials include seizures and essential tremors.
A derived trait is a trait that the current organism has, but the previous one did not have.An ancestral trait is a trait that you, and your ancestors had.A vestigial structure is a structure that had some sort of use in your ancestor, but none in your current body
Sodium is kept immersed in kerosene because Sodium doesn't react with kerosene.
It only can focus on high powers
