Download the app called Socratic it helps with these questions
Answer: B. Melting temperatures of primer should be between 55-80 degree Celsius.
Explanation:
Bacause the melting temperature controls the binding of the primers to your template DNA. At melting temperature 50% of the primer molecules are bound to their corresponding target sequence. If the difference in melting temperature between the two primers is too high, it might be difficult to find experimental conditions where both primers can bind to their target.
One long, single molecule of double stranded, helical, supercoiled DNA. In most bacteria, the two ends of the double-stranded DNA covalently bond together to form both a physical and genetic circle
The answer is hamstrings!! hope this helped. have a nice day:)
Trait
I'm sorry I don't know how to explain it.
