The DNA replication products visualized during the sanger method of DNA sequencing are observed in which nucleotides are added.
Sanger sequencing is based on the process of DNA replication. A scientist creates a copy of his DNA strand. Then observe which nucleotides have been added. This way you can see the sequence of nucleotides. A laser excites the fluorescent labels in each band and a computer detects the resulting light.
Sanger sequencing produces extension products of various lengths ending in dideoxynucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer. Selective incorporation of chain-terminating dideoxynucleotides by DNA polymerases during in vitro DNA replication.
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They once thought that humans came from monkeys and apes but now they think they come from a ancient ancestor not from the monkey or ape family.
Explanation:
Groundwater is defined as water that is found beneath the surface of the earth in conditions of 100 percent saturation (if is less than 100 percent saturation,then the water is considered soil moisture).Ninety-eight percent of earth’s available fresh water is groundwater
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