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The DNA replication products visualized during the sanger method of DNA sequencing are observed in which nucleotides are added.
Sanger sequencing is based on the process of DNA replication. A scientist creates a copy of his DNA strand. Then observe which nucleotides have been added. This way you can see the sequence of nucleotides. A laser excites the fluorescent labels in each band and a computer detects the resulting light.
Sanger sequencing produces extension products of various lengths ending in dideoxynucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer. Selective incorporation of chain-terminating dideoxynucleotides by DNA polymerases during in vitro DNA replication.
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Cell division is the process by which a parent cell divides into two or more daughter cells.[1]Cell division usually occurs as part of a larger cell cycle. In eukaryotes, there are two distinct types of cell division: a vegetative division, whereby each daughter cell is genetically identical to the parent cell (mitosis),[2] and a reproductive cell division, whereby the number of chromosomes in the daughter cells is reduced by half to produce haploid gametes(meiosis). Meiosis results in four haploid daughter cells by undergoing one round of DNA replication followed by two divisions. Homologous chromosomes are separated in the first division, and sister chromatids are separated in the second division. Both of these cell division cycles are used in the process of sexual reproduction at some point in their life cycle. Both are believed to be present in the last eukaryotic common ancestor.
Prokaryotes (bacteria) undergo a vegetative cell division known as binary fission, where their genetic material is segregated equally into two daughter cells. All cell divisions, regardless of organism, are preceded by a single round of DNA replication.
For simple unicellular microorganisms such as the amoeba, one cell division is equivalent to reproduction – an entire new organism is created. On a larger scale, mitotic cell division can create progeny from multicellular organisms, such as plants that grow from cuttings. Mitotic cell division enables sexually reproducing organisms to develop from the one-celled zygote, which itself was produced by meiotic cell division from gametes. After growth, cell division by mitosis allows for continual construction and repair of the organism.[3] The human body experiences about 10 quadrillion cell divisions in a lifetime.[4]
The primary concern of cell division is the maintenance of the original cell's genome. Before division can occur, the genomic information that is stored in chromosomes must be replicated, and the duplicated genome must be separated cleanly between cells.[5] A great deal of cellular infrastructure is involved in keeping genomic information consistent between generations.
