Answer:
By way of introduction, A260/A280 ratio is use to measure Protein Contamination. This procedure was first described to measure protein purity in the presence of nucleic acids. However it is now commonly used to assess protein contamination of DNA. The method to determine the concentration of protein contamination by using A260/280 ratio method is well explained below by filling in the missing words.
Explanation:
The A260/A280 ratio method estimates protein __purity___ by measuring the absorbance maximum at 280nm caused by the amino acids__cytosine___, ____Adenine____, and ___Guanine_________ Since ___Spectrophotometer____ also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at ____260nm______ and using the following equation: Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml___ When extrapolated from a standard curve, the Bradford data indicates the amount (in _ug__ ) of __unknown ___ protein found in a sample. If you know the volume of sample that was added to the assay then you can calculate the protein ____concentration ___ of the sample. The coomassie blue dye in the Bradford assay specifically binds primary ¬¬___sulfonic___ and __positive amines__ groups of the amino acid side groups of the proteins. The more the dye binds to the sample, the _anionic_ the blue color will be, and the absorbance at 595¬nm will be_shifted Amax___. A sample with an unusually __protein___ number of ___280nm of Tyrosine __ [give a specific example] amino acids will underestimate the total amount of protein present in the sample.
Answer:
The geocentric model says that the earth is at the center of the cosmos or universe, and the planets, the sun and the moon, and the stars circles around it. The early heliocentric models consider the sun as the center, and the planets revolve around the sun.
Explanation:
Answer:
C.
Explanation:
The small population size means that the new colony may have reduced genetic variation from the original population and that variation, in this case, included the dominant allele for Huntington's disease. Genetic drift, along with natural selection, mutation, and migration, is one of the basic mechanisms of evolution. In this case, it was a chance that the settlers all carried the allele for Huntington's disease.
Answer:
tundra, taiga, temperate grassland, rainforest
Explanation:
Tundra is known to be the coldest of all the biomes due to the high latitudes in which these areas are found. In this biome, the growth of the trees is limited by the low temperatures and the growing season is restricted to the summer season. The taiga is a type of biome composed of forest found in the subarctic areas, it is for that reason that taiga regions are also called boreal forests. Temperate grasslands are areas where grasses are dominant with very variable temperatures depending on the season (i.e. summer to winter seasons). In this biome, trees are relatively uncommon. Finally, the rainforest is the most diverse tree-rich biome where the high temperature and humidity conditions are optimal for life.
