10 things I know about you:
1. You are reading this
2. You are a human
3. You can't say the letter "p" without separating your lips
4. You just attempted to do it
6.You are laughing at yourself
7. You have a smile on your face and skipped number 5.
8. You just checked to see if there was a number 5.
9. You laugh at this because you fell for it and everyone else did too.
10. Now copy and paste this to see who else falls for it! # i know what you doing.
Answer:
Disulfide linkage/bond
Explanation:
The tertiary and quarternary structure of the protein is stabilized by Disulfide linkage which is formed between two thiol groups in the protein. 2-mercaptoethanol is a reducing agent that breaks this disulfide bond.
A protein becomes denature when it loses its native configuration and becomes inactive. So as 2-mercaptoethanol breaks disulfide bond in protein it looses its native configuration and becomes denatured. 2-mercaptoethanol is used in SDS-PAGE to separate protein subunits. Therefore the correct answer is Disulfide linkage/bond.
