Answer:
The smallest size tube that will hold the entire reaction is 0.5ml
Explanation:
25ul (DNA sample) + 25ul (enzyme) + 50ul (buffer) + 400ul (water) = 500ul total
since 1000ul=1ml, then 500ul = 0.5ml
Answer:
The device that mostlikely would not be used P20
Explanation:
P20 is a pipette used to measure and sample volumes of 20ul maximum.
Answer:
The reaction mix will have 400 units of the enzyme.
The working dilution will be 0.8 Units/ul.
Explanation:
The mix has 20ul of the enzyme which is 20Units/ul. Then we have 20ulx20Units/ul= 400 Units of enzyme in the mix.
Since the final volume is 500ul, the final concentration 400Units/500ul=0.8 Units/ul.
I’m pretty sure it’s sperm
Answer:
The correct answer would be a lipid-soluble signal.
Signal transduction refers to the set or cascade of bio-chemical reactions take place in a cell in order to transmit the chemical or physical signal from outside the cell to inside target molecule.
It is usually initiated by receptors present on the membrane of the cell which sense the extracellular stimulus (such as ligand, hormone, pressure, temperature, pressure etc). Once activated, these receptors activate the cascade of reaction which ultimately reaches the target molecule and produces a cell's response.
In contrast, the lipid-soluble signals (such as steroidal hormones, thyroid hormone etc) enter the cell membrane without any hindrance. They directly bind to intra-cellular receptor present in the cytoplasm or on the nuclear membrane.
The hormone-receptor complex then enters the nucleus and act as transcription factor and produces the response in the form of transcription.
A specises that is very close to extinction that they are trying to save