Question

Assume that one of the disinfectant/antiseptics tested using the disc method had a clear zone of inhibition and to your surprise, you discover minute growth near the disc. What would explain this phenomenon

Answer 1

The presence of the minute growth near the disc signifies that the bacterial innoculum contains mutant microorganisms that are able to grow in the presence of toxic substances.

What are zones of inhibition?

The sensitivity testing in a microbiology laboratory is done to determine the effect of antibiotics on various bacteria organisms

The zone of inhibition when a disk method is used signifies the area of media where bacteria are unable to grow, due to presence of a drug that impedes their growth.

If there are presence of minute growth near the disc, it means that the bacterial innoculum contains mutant microorganisms that are able to grow in the presence of toxic substances such as disinfectants.

If these mutant bacteria organisms are not cleared by the disk method, it means that they cannot be killed by the disinfectant/antiseptics that is being tested.

Learn more about antiseptics here:

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