Question

How is a cesium gradient used to separate dna with different densities?

Answer 1

Cesium is used in the separation of DNA with different densities. Cesium chloride (CsCl) acts as a density gradient for the separation of the DNA (Deoxy ribonucleic acid), and is used in gradient centrifugation.

CsCl density centrifugation:

• CaCl solution is kept in the centrifuge tube along with the DNA sample to be separated.
• The centrifugation causes the solutions to form bands with the denser part at the bottom and the least denser part at the top.
• The DNA with its density similar to a particular layer of CsCl solution density forms a band in that layer.

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