Answer:
The article from a primary scientific literature uses a mycobacterial group as a model organism for researchers to determine where they get their extra nutrients in the hostile environments they infect.
The study, which was supported by the Australian Research Council (ARC) and National Health and Medical Research Council (NHMRC), was published in the ISME Journal.
a. why did the scientist perform the study?
b. What was then hypothesis under investigation?
c. what were thhe major results and did they support or negate the hypothesis?
d. Which Key technique
Explanation:
A microbe as a model organism would be considered as a proxy organism to lead researchers to get the answers on human´s health enigmas due to the fact that several of their facets are similar to ours, so investigations on these model organisms are highly helpful to understand our own biology.
a. This is a study on a mycobacterial group responsible for diseases as tuberculosis, leprosy, Buruli ulcer, and the scientists performed it to determine what this bacteria do or how they obtain the nutrients they need to survive while they are on an adverse setting, which is during the infection.
b. The hypothesis under investigation stated that carbon monoxide consumption was the responsible for mycobacteria to survive, without growth though, but enough to survive.
c. The results supported the hypothesis that an enzyme known as carbon monoxide dehydrogenase gas is what provides mycobacteria with energy for longer periods of time as they might be able to survive inside the human by using carbon monoxide.
d. The technique used by researchers were a considerable amount of mycobacteria cultures on carbon monoxide to observe if the bacteria thrived on it using this gas as an energy source, and cultures without it and observe if the survived.
"When microbial cells are starved of their preferred energy sources, one way they subsist is by scavenging gases such as carbon monoxide," said Monash PhD student Paul Cordero, the co-lead author of the study.
"They breakdown this gas into its fundamental components, which provide the cells just enough energy to persist."
"Based on these findings, we predict that it uses this gas to its advantage to persist inside human lungs,"
"Our immune cells actually make small amounts of carbon monoxide, which the bacterium may be able to use as an energy supply while dormant."
