Answer:
the layer at the bottom of the glass
Explanation:
because the last layer consists with so much energy so all the force is in the last layer.
Hi if it is wrong am sorry I tried but if it is right then am happy to help.
Answer:
Optical trapping interferometry
Explanation:
Optical trap is a device which can measure forces or dielectric objects of magnitude of Pico newton or micron.
In this, a high focused light beam is used to view objects under the microscope. It is highly used in molecular and cell biology and biophysics
This methodology has been adapted to sort lipids at cell surface and in activation of kinase in the dendritic cells
Answer:
One significant difference that I noticed was the color of the water. In the control aquarium, the color of the water at 30 days was the same as it was on the 1st day. In the experimental aquarium, the water had a green tint on the 30th day, but on the first day it was clear.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
proteins is the answer of this question
