Hey there mate!
Based on my information to this, I believe sense (it would be a closed system), this would most likely be considered to be (equal to) the total energy after.
But, if it was not a (closed system), it would not be unrelated because the energetic system would not be that greatly increased.
So, it really depends whether the system closes up, or whether it would be free of energy.
So, in this case, your correct option to this answer would be (option A).
<span>In a closed system, the total energy prior to an energy transformation is unrelated to the total energy after.
I hope this helps you!</span>
Cytoplasm. This photo should help explain it!
I am not entirely sure what you mean by "responding variable" but I'm going to guess it was the vinegar since it has been used as a perservative for years.
Hope that helps in some way.