Two limits to cell growth is by rates of protein synthesis and by the folding rates of its slowest protein
Answer:
subluxation.
Explanation:
Subluxation is the partial displacement of a bone from its joint.
Dislocation is complete displacement of a bone from its joint.
Luxation is complete separation of joints.
Sublocation generally return to its normal position without the help from orthopedic doctor.
Example; Nursemaid's elbow caused by subluxation of head of radius from annular ligament.
Answer:Igneous rocks are made from cooling of lava or magma.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
They have increased need to carbohydrates, protein, water and iron.
Carbohydrates is the primary source for energy and active people apparently need more energy for their daily activities.
Protein helps build muscles, even though they only need a small amount more of protein than normal people, they still require protein for muscle buildings.
Water has to be needed as they may excrete more sweat by active activities in the day time. Water can also help remove waster material from body.
Iron helps the production of hemoglobin, which is in the red blood cells that help transport oxygen. As they exercise more, they need more oxygen supply for respiration, therefore there is a need for iron supply.
