Question

A separation technique that can detect proteins in a complex mixture with the use of antibodies directed against a protein of interest is

Answer 1

Northern blotting is the separation technique that can detect proteins in a complex mixture with the use of antibodies directed against a protein of interest.

Commonly used protein separation techniques include ion exchange chromatography, affinity chromatography, dialysis, ultrafiltration, size exclusion chromatography, and electrophoresis [sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Isoelectric point electrophoresis, and chromatographic electrophoresis] are included.

Northern blot is a laboratory analysis method for testing RNA. In particular, purified RNA fragments are separated from biological samples (such as blood and tissue) by passing through a gel or matrix like a sieve with an electric current. This allows small fragments to move faster than large fragments.

Northern blotting is named because it resembles Southern blot, the first blotting technique named after biologist Edwin Southern. The main difference is that Northern blots analyze RNA rather than DNA.

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