If you suspect your culture of bacteria has 23 x 106 cells per mL, what would you want the final dilution to be in order to end
1 answer:
The dilution factor in order to end up with 96 colonies of bacteria is 0.23 × .
For calculating the colony forming unit bacteria;
Colony-forming unit (CFU or cfu) is a measurement of live bacterial or fungi cells and is used to calculate the colony-forming unit of bacteria. CFU counts only viable cells as opposed to direct microscopic counts, which count all cells, both living and dead, using a hemocytometer. Colony-forming units per milliliter (CFU/mL) for liquids and colony-forming units per gram (CFU/g) for solids are used to indicate the results for convenience. The miles and Misra method can be used to compute CFU, which is helpful for assessing the microbiological load and severity of illness in blood and other samples.
∴ cfu/ml = (no. of colonies x dilution factor) / volume of culture plate
23 x cells per mL = 96 × dilution factor
Dilution factor = 23 × / 96
Dilution factor = 0.23 ×
Therefore, the dilution factor in order to end up with 96 colonies of bacteria is 0.23 × .
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