Question

Photodecomposition, photomutagenicity and photocytotoxicity of retinyl palmitate under He-Ne laser photoirradiation and its effects on photodynamic therapy of cancer cells in vitro

Answer 1

The effects on photodynamic therapy of cancer cells in vitro is that retinyl palmitate (RP) can be used 24 h before PDT to enhance its effects. RP is not mutagenic under irradiation with He–Ne laser.

HeLa cells with or without RP (retinyl palmitate) that was incubated with the cells for a short or long incubation period prior to PDT are typically used to test the effects of PDT (photodynamic therapy) using hematoporphyrin derivatives (HpD) as photosensitizer under He-Ne laser irradiation.

No photodecomposition of RP alone is visible, but a small amount is seen of in the presence of HpD under He-Ne laser irradiation is seen. No photomutagenicity is often seen under laser irradiation. HeLa cell viability can be dramatically reduced by RP alone. A 24 hour incubation period before PDT increased the cytotoxicity of PDT on HeLa cells.

Therefore, retinyl palmitate (RP) can be used 24 h before PDT to enhance its effects. RP is not mutagenic under irradiation with He–Ne laser.

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