Answer:
DNA ligase,joins the okazaki fragments together into a single DNA molecule
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
This evidence suggest that all three living creatures that are mentioned have a common ancestor, even though they have been split into different types of groups of living organism millions of years ago.
Science suggests that all of the living organisms on the planet have a common ancestor. Since we are talking about a common ancestor that lived few hundred million years ago, the living organisms split into different groups, and they do not resemble each other nowadays. The fruit fly is an insect, and the humans and mice are mammals, so they are into totally different groups, and it is easy to see by the genetic evidence that they are very distantly related, but the humans and mice are much more closely related because they belong to the same super family, and their connection is much more recent.
Stay inside!
If you can't find shelter stay in your car but do not stand underneath a tree for fear of lightning!
The segments of DNA that code for specific traits are called genes
