Question

Addition of a nucleotide onto a dna strand is an endergonic reaction. what provides the energy to drive the reaction?

Answer 1

The release of pyrophosphate from the incoming nucleotide, and then hydrolysis of the pyrophosphate to inorganic phosphate provides energy for the addition of nucleotide onto a DNA strand.

Nucleotides are linked together by a condensation event that yields a tiny, stable molecule. But the released molecule is pyrophosphate, not water. A good amount of free energy is released when water is added to pyrophosphate.

The high-energy link between the ejected beta and gamma phosphates stores the energy for each incoming nucleotide's addition. The subsequent hydrolysis that occurs drives the process. A substantially greater quantity of energy is released when two phosphates are separated into individual phosphates.

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