Question

You are preparing to use a reverse transcriptase to create cDNA from a sample containing purified mature eukaryotic mRNA. If you are preparing a primer using only one type of nucleotide, the primer most likely to be successful would be

Answer 1

To prepare a primer using only one type of nucleotide for the formation of cDNA, the primer most likely to be successful would be oligo-dT primer.

A primer is a short stretch of nucleotides of either DNA or RNA that bind to the stretch of DNA and also help in its identification.

Oligo-dT primers contain a short stretch of deoxythymidines. It is the most preferred type of primer for synthesizing the cDNA using reverse transcriptase. They have very high specificity and also allows to study various gene pools all simultaneously. They are suitable for m-RNAs containing a poly-A tail in them.

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