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Norma-Jean [14]
2 years ago
6

You are preparing to use a reverse transcriptase to create cDNA from a sample containing purified mature eukaryotic mRNA. If you

are preparing a primer using only one type of nucleotide, the primer most likely to be successful would be
Biology
1 answer:
stiks02 [169]2 years ago
4 0

To prepare a primer using only one type of nucleotide for the formation of cDNA, the primer most likely to be successful would be oligo-dT primer.

A primer is a short stretch of nucleotides of either DNA or RNA that bind to the stretch of DNA and also help in its identification.

Oligo-dT primers contain a short stretch of deoxythymidines. It is the most preferred type of primer for synthesizing the cDNA using reverse transcriptase. They have very high specificity and also allows to study various gene pools all simultaneously. They are suitable for m-RNAs containing a poly-A tail in them.

To know more about primers, here

brainly.com/question/14698863

#SPJ4

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What is the new peptide chain when the new dna segment is translated for 3'- TACAGGGTGCTACCCACT-5'
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Methionine- Serine- Histidine- Aspartate- Glycine- stop

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Translation, which occurs in the ribosomes, is the second stage of protein synthesis where mRNA transcript is used to synthesize a peptide chain that eventually forms a protein. The mRNA sequence is read in a group of three nucleotide called CODON. Each codon specifies a particular amino acid, which is read and added to the growing peptide chain.

In this question, a DNA sequence reds 3'- TACAGGGTGCTACCCACT-5, the

mRNA sequence from this DNA during transcription will be:

AUG UCC CAC GAU GGG UGA

From this mRNA sequence, the peptide chain that will result following translation is:

Methionine- Serine- Histidine- Aspartate- Glycine- stop

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