Name of the method to test the hypothesis that the bacteria can be transformed with the heat-killed ampicillin resistant supernatant is DNA - Transformation Experiment.
<h3 /><h3>Why Ampicillin used in bacterial transformation ?</h3>
Ampicillin is an antibiotic which stops the E. coli from building cell walls, killing the bacteria in the process. An enzyme that prevents the effects of ampicillin is produced when the ampicillin-resistance gene is active, allowing the bacteria to survive.
Only transformation bacteria which contain plasmid and can make the beta lactamase can survive on plates which contain ampicillin. Untransformed cell cannot grow on the plates that contain ampicillin.
Reason behind the resistance ; The -lactamase enzyme, which cleaves the -lactam ring of ampicillin, is the root cause of ampicillin resistance. The resistance gene known as bla gene produces this -lactamase enzyme.
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