Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry

1 answer:

5 0

Exosomes are small vesicles of size 40-90 nm. They are released by antigen presenting cells. According to recent research, exosomes have shown to play a role in cell-cell communication.

Exosomes have a high degree of MHC Class II expression because they come from MHC class II peptide loading compartments. Three distinct and highly polymorphic isotypes, HLA-DR, -DQ, and -DP, respectively, encode the human MHC class II.

For the purpose of precisely isolating exosomes from cell-free supernatants, magnetic beads have been coated with monoclonal antibodies specific for HLA DP, DQ, and DR.Different molecules can be purified using magnetic beads, which is an easy and dependable technique.

Exosome-coated beads are then stained with conjugated antibodies and subjected to flow cytometric analysis. This approach can characterize exosomes revealing MHC Class I and II molecules and their associated cells.

To learn more about exosomes click here

brainly.com/question/14285538

#SPJ4

You might be interested in

Label the plant elements

spin [16.1K]

Explanation:

everything can be found in the picture

3 0

3 years ago

Distinguish a virus from a prophage.

vazorg [7]

$\: \: \: \huge \color{blue} \boxed{ \colorbox{black}{Answer}}$

A prophage is a bacteriophage (often shortened to "phage") genome inserted and integrated into the circular bacterial DNA chromosome or exists as an extrachromosomal plasmid. This is a latent form of a phage, in which the viral genes are present in the bacterium without causing disruption of the bacterial cell.

▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬

6 0

2 years ago

What is the outcome for earth after the moon rotates around it?

borishaifa [10]

The Moon appears to Earth as a small crescent, as it rotates around the Earth this expands to a full moon and back to a small crescent. This happens once every 29 days and is called a lunar month. The Moon causes the rise and fall of the ocean's tides on Earth.

6 0

3 years ago

Biotic factors in the environment include ???

Anon25 [30]

Biotic factors are anything in a ecosystem that is living, was living, or is going to live.

From this we can conclude that the biotic factors are remains of dead organisms and flora and fauna.
Temperature and minerals in the soil are abiotic, meaning they will never live.

6 0

3 years ago

Read 2 more answers

How does the Digestive system help maintain homeostasis?

Gnesinka [82]

Answer:

The bacterial flora in the intestines are essential to homeostasis in the body, they not only break down food so the nutrients can be absorbed, they produce vitamins like biotin and vitamin K and guard against harmful bacteria that enter the system. While your heart is a vital organ, the brain (and the nervous system that attaches to the brain) make up the most critical organ system in the human body. The digestive system ordinarily gets 20% to 25% of the oxygenated blood pumped out by the heart and the receptors in muscles provide the brain with information about body position and movement, the brain controls the contraction of skeletal muscle the nervous system regulates the speed at which food moves through the digestive tract.

8 0

3 years ago