1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
elena-s [515]
2 years ago
11

Development of a multiplex real-time PCR assay for the simultaneous detection of four bacterial pathogens causing pneumonia

Biology
1 answer:
Ainat [17]2 years ago
4 0

To simultaneously identify the four bacterial pathogens that cause pneumonia, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and Moraxella catarrhalis, a multiplex real-time polymerase chain reaction (mRT-PCR) technique was developed.

Limit of detection (LOD), specificity, and reproducibility were used to assess the analytical performance of mRT-PCR against target pathogens. For the "respiratory bacteria four" (RB4) mRT-PCR assay, 210 clinical samples from pneumonia patients were processed using automatic nucleic acid extraction equipment.

The outcomes were then directly compared to references from bacterial culture and/or Sanger sequencing. With a coefficient of variance ranging from 0.29 to 1.71 and a cautious LOD of DNA equating to 5 102 copies/reaction, the RB4 mRT-PCR test identified all target pathogens from sputum samples.

Additional bacterial infections were found in reference-negative specimens, and the assay had 100% agreement with reference-positive samples.

learn more about pathogens brainly.com/question/27775291

#SPJ4

You might be interested in
_____ was a Key technique used to explain the structure of DNA.
ValentinkaMS [17]

Answer:

X-ray crystallography

Explanation:

3 0
3 years ago
Read 2 more answers
1. The following statement is similar to those you might see on exams. Write a paragraph of 3-5 sentences describing 1) whether
yanalaym [24]

Answer:

The oxygen dissociation curve represents the percentage saturation of Hb with oxygen at different partial pressure of oxygen. The different partial pressures gives sigmoid shapes to the curve. When this curves shifts to right, it  indicates  low affinity or binding of oxygen by the Hb. it also indicates the unloading or releases of Oxygen by Hb molecules at condition of low pressure. e,g in the muscles during strenuous exercise.However, when the curve shifts to the left, this indicate high affinity for oxygen, great binding, at high partial pressure of oxygen.e,g in the lungs to take oxygen and releases CO2.

Therefore in this scenario, the statement -. <u>During strenuous exercise, the oxygen-hemoglobin dissociation curve shifts to the right.</u> is correct. because oxygen is needed by the muscles therefore ,oxygen should be less binded by Hb,  decrease affinity and easily unloaded to muscles.

<u>The statement </u>This rightward shift reflects an increase in the affinity of hemoglobin for oxygen and favors loading of O2 into hemoglobin in the lungs is wrong.

As explained above the rightwards shift indicated low affinity of Hb for oxygen(unloading)and  favours unloading at the muscles because during strenuous exercise the partial pressure of oxygen is very low(but that of CO2 high) in the muscles which favours low oxygen molecules  binding by Hb, and easy release to respiring cells.

Explanation:

4 0
3 years ago
explain why the number of squid might change if there were a significant decrease in the number of cod
marusya05 [52]
More elephant seals and peguins would be eating (killing) the squids. Also it might not realy change at all because the squid has much more krill and small animals to eat.
7 0
3 years ago
Would someone be willing to help me out with my English and Language Arts questions. Need to have them done today. Please only a
Rainbow [258]
What are the questions
3 0
4 years ago
Name one type of substance that is taken into a root hair cell using active transport.
slamgirl [31]

Answer:

The substance taken in is mineral salts.

5 0
3 years ago
Other questions:
  • Which phrase best describes genetics? A the science of life B the science of heredity C the science of reproduction D the scienc
    15·2 answers
  • Help me answer this question please.
    10·1 answer
  • What is the defintion of handicraft
    8·2 answers
  • A population of black cats has been interbreeding. However, in one litter, a sole gray kitten is born. The successive generation
    7·2 answers
  • How does the human heart organization relate to cells? To organ systems?
    11·1 answer
  • What do we call the rugged, broken up parts of the surface of the moon that appear to be brighter colors
    6·2 answers
  • NEED THIS ANSWER ASAP<br><br><br> How do humans develop from gametes
    13·1 answer
  • There is more then one answer to the question. What does the brachiocephalic divide into? There is more thane one answer to the
    5·1 answer
  • 40 points! PLEASE HELP!!!
    14·1 answer
  • Important long-term contributions of ancient Greek and Roman civilizations are primarily found in the area of religious doctrine
    5·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!