1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
elena-s [515]
2 years ago
11

Development of a multiplex real-time PCR assay for the simultaneous detection of four bacterial pathogens causing pneumonia

Biology
1 answer:
Ainat [17]2 years ago
4 0

To simultaneously identify the four bacterial pathogens that cause pneumonia, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and Moraxella catarrhalis, a multiplex real-time polymerase chain reaction (mRT-PCR) technique was developed.

Limit of detection (LOD), specificity, and reproducibility were used to assess the analytical performance of mRT-PCR against target pathogens. For the "respiratory bacteria four" (RB4) mRT-PCR assay, 210 clinical samples from pneumonia patients were processed using automatic nucleic acid extraction equipment.

The outcomes were then directly compared to references from bacterial culture and/or Sanger sequencing. With a coefficient of variance ranging from 0.29 to 1.71 and a cautious LOD of DNA equating to 5 102 copies/reaction, the RB4 mRT-PCR test identified all target pathogens from sputum samples.

Additional bacterial infections were found in reference-negative specimens, and the assay had 100% agreement with reference-positive samples.

learn more about pathogens brainly.com/question/27775291

#SPJ4

You might be interested in
Name the organelle that perfoms each of the following function in acell protain synthesis​
vladimir1956 [14]

Answer:

Ribosomes

Explanation:

hope it helps

5 0
3 years ago
The __________ is the main control center for the autonomic nervous system. A. forebrain B. thalamus C. hypothalamus D. cerebrum
nirvana33 [79]
The autonomic nervous system (ANS) is the part of the nervous system which is involuntary. It is composed of neurons that is responsible for impulsing the central nervous system to muscles and glands. The hypothalamus is the most important part and main control center of ANS. It is responsible for many homeostatic processes inside our body. Both the autonomic function and the endocrine function is regulated by the hypothalamus.
3 0
3 years ago
Read 2 more answers
How does indicate that a. dog is more closely related to a red fox than a house cat?
Lelechka [254]

<u>Answer:</u>

Dog is more closely related to a red fox than a house cat according to a taxonomy level.

<u>Explanation:</u>

  • Dog, red fox, house cat is in the same kingdom Animalia, same phylum Chordata, same class Mammalia, same order Carnivora but the family of red fox and the gad is same i.e. Canidae but the family of the house cat is Felidae.
  • The red fox and dog have more taxon in common. The house cat diverges at the family level.
3 0
3 years ago
Explain with scientific reason that origin of life was associated with some chemical reactions.​
Hunter-Best [27]

Answer:

Darwin's theory of biological evolution tells us that all life on earth may have originated from a single, relatively simple reproducing creature living in the distant past. This idea is based on many observations, one of which is that when living things reproduce, children are often born with random new traits.

Explanation:

5 0
2 years ago
1. If a lily (a type of flower) has 48 chromosomes in its leaf cells, how many chromosomes will its pollen have?
stepan [7]

Answer:

i want to say 6 but I am not sure

4 0
3 years ago
Other questions:
  • Which of the following structures is where cellular respiration occurs in a cell
    7·1 answer
  • The __________ is a fluid structure that allows membrane proteins to move freely.
    8·2 answers
  • Which type of chromosomal mutation causes Klinefelter syndrome? nondisjunction translocation deletion insertion
    7·2 answers
  • "Briefly DISCUSS four (4) instances in which taking any vitamin and mineral supplements are unnecessary, or even harmful. Do NOT
    15·1 answer
  • In which culture is a young adolescent given instructions about how to please a girl and bring her to orgasm?
    14·1 answer
  • Describe the channels and ions that neurons use to generate an action potential. (include when channels open and close and which
    12·1 answer
  • SOMEONE I NEED HELP!!
    5·1 answer
  • The question is in the picture
    8·1 answer
  • What is photosynthesis ??????​
    5·2 answers
  • In the real world, population explosion is usually followed by Select one: a. A continuation of high population levels indefinit
    9·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!