Question

Why it is important to place the gel in the correct direction in the gel cassette while running it?

Answer 1

The gel should be placed in the correct direction so that the DNA bands can move.

• DNA is negatively charged due to the presence of phosphate groups, so it can only move towards the positively charged anode, otherwise, it will not move.
• So, when gel electrophoresis is done the side of the gel where the wells have been created and DNA samples have been loaded is placed at the side of the cathode, whereas the other side is connected to the anode.
• When the current flows, the DNA fragments also move from negatively charged cathode to positively charged anode as per their mass and size.

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