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sertanlavr [38]
2 years ago
12

What is the specific application of reverse transcriptase in the preparation of cdna?

Biology
1 answer:
DiKsa [7]2 years ago
4 0

The specific application involved in reverse transcriptase preparation of cDNA is a synthesis of DNA to form an RNA-DNA duplex.

What is reverse trancriptase activirt in cDNA?

  • Recombinant RNA-dependent DNA polymerases known as reverse transcriptases are employed in molecular biology to catalyze the conversion of RNA into DNA during a first-strand cDNA synthesis reaction.
  • A double helix molecule with an RNA-DNA hybrid is created by this process. When an RNA fragment is complexed with DNA, reverse transcriptases feature an RNase H domain with endoribonuclease activity that specifically breaks down RNA.
  • The RNA fragment can only be transcribed once thanks to the degradation of RNA in an RNA-DNA complex, which minimizes transcription bias and ensures reaction fidelity.

Reverse transcriptases from either the avian myeloblastosis virus (AMV) or the Moloney murine leukaemia virus (MMLV) are used in the majority of commercial reverse transcription kits.

Compared to AMV, MMLV provides a number of benefits, but its reduced error rate and greater processivity rate stand out.

Hence, the correct answer is a synthesis of DNA to form an RNA-DNA duplex.

Learn more about RNA here,

brainly.com/question/20914096

# SPJ4

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An enzyme is a catalysts in living things.

A catalysts speeds up the process of breaking down molecules.

An example would be Lactose. It can be broken down by the enzyme Lactase.

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Where is the least amount of energy transferee in a food web ?
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Answer:

The least amount of energy is at the very told of the food web.

Explanation:

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How does random fertilization add to the genetic variation?
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3 years ago
Part A Which of the following statements regarding the umbilicus and the chin is true?
garik1379 [7]
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3 years ago
If you were to expose cells that are undergoing aerobic cellular respiration to a radioactive oxygen isotope in the form of O2,
HACTEHA [7]

Answer:

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For the purpose, NADH, and FADH2 transfer their electrons to the molecular oxygen via electron transport chain. After accepting the electrons, molecular oxygen is oxidized into the water molecule. Therefore, radioactive oxygen isotope would appear in the form of a water molecule after completion of cellular respiration.

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