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sertanlavr [38]
2 years ago
12

What is the specific application of reverse transcriptase in the preparation of cdna?

Biology
1 answer:
DiKsa [7]2 years ago
4 0

The specific application involved in reverse transcriptase preparation of cDNA is a synthesis of DNA to form an RNA-DNA duplex.

What is reverse trancriptase activirt in cDNA?

  • Recombinant RNA-dependent DNA polymerases known as reverse transcriptases are employed in molecular biology to catalyze the conversion of RNA into DNA during a first-strand cDNA synthesis reaction.
  • A double helix molecule with an RNA-DNA hybrid is created by this process. When an RNA fragment is complexed with DNA, reverse transcriptases feature an RNase H domain with endoribonuclease activity that specifically breaks down RNA.
  • The RNA fragment can only be transcribed once thanks to the degradation of RNA in an RNA-DNA complex, which minimizes transcription bias and ensures reaction fidelity.

Reverse transcriptases from either the avian myeloblastosis virus (AMV) or the Moloney murine leukaemia virus (MMLV) are used in the majority of commercial reverse transcription kits.

Compared to AMV, MMLV provides a number of benefits, but its reduced error rate and greater processivity rate stand out.

Hence, the correct answer is a synthesis of DNA to form an RNA-DNA duplex.

Learn more about RNA here,

brainly.com/question/20914096

# SPJ4

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Even though rodents known as sugar gliders and flying squirrels are members of distinctly different groups of organisms and live
EleoNora [17]

Answer:

convergent evolution.

Explanation:

When organisms of distantly related species or groups are found in similar environmental conditions, they develop some similar features which are otherwise not present in their ancestral species. This is called convergent evolution. Convergent evolution also results in the development of similar traits in the distantly related organisms that are found in distantly located geographical regions having similar environmental conditions. Therefore, the presence of the same features in rodents and flying squirrels represent convergent evolution.

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3 years ago
What kind of mutation would occur if a sequence of DNA is changed from ATCGGCTTAACG to ATCGGCTAAACG?
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Answer:

Base substitution mutation

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ATCGGCTTAACG to ATCGGCTAAACG

This type of mutation is called SUBSTITUTION MUTATION. Substitution mutation is a type of mutation that involves the replacement of one or more nucleotide base by another. In this original sequence; ATCGGCTTAACG, the eight base T was replaced by base A in the sequence, hence, it is a substitution mutation.

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