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2 years ago

What is the specific application of reverse transcriptase in the preparation of cdna?

1 answer:

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The specific application involved in reverse transcriptase preparation of cDNA is a synthesis of DNA to form an RNA-DNA duplex.

What is reverse trancriptase activirt in cDNA?

Recombinant RNA-dependent DNA polymerases known as reverse transcriptases are employed in molecular biology to catalyze the conversion of RNA into DNA during a first-strand cDNA synthesis reaction.
A double helix molecule with an RNA-DNA hybrid is created by this process. When an RNA fragment is complexed with DNA, reverse transcriptases feature an RNase H domain with endoribonuclease activity that specifically breaks down RNA.
The RNA fragment can only be transcribed once thanks to the degradation of RNA in an RNA-DNA complex, which minimizes transcription bias and ensures reaction fidelity.

Reverse transcriptases from either the avian myeloblastosis virus (AMV) or the Moloney murine leukaemia virus (MMLV) are used in the majority of commercial reverse transcription kits.

Compared to AMV, MMLV provides a number of benefits, but its reduced error rate and greater processivity rate stand out.

Hence, the correct answer is a synthesis of DNA to form an RNA-DNA duplex.

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Rainbow [258]

I think A. Y but honestly not so sureee

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3 years ago

T or f sound waves can travel across outer space?

elena-14-01-66 [18.8K]

Answer:

False

Explanation:

Sound does not travel at all in space. The vacuum of outer space has essentially zero air. Because sound is just vibrating air, space has no air to vibrate and therefore no sound. ... Radio is a form of electromagnetic radiation just like light and can therefore travel through the vacuum of space just fine.

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3 years ago

7. White wool (WW, Ww) and brown eyes (BB, Bb) are dominant traits in sheep, while black wool (ww) and blue eyes (bb) are recess

artcher [175]

Answer:

1/2 wWBb , White wool and brown eyes.

1/2 wWbb , White wool and blue eyes.

Explanation:

White wool (W) is dominant over black wool (w).

Brown eyes (B) is dominant over blue eyes (b).

A sheep with black wool (ww) and brown eyes (Bb) is crossed with a sheep with white wool (WW) and blue eyes (bb).

If there is independent assortment between genes, the genotypes of the first parent's gametes will be wB and wb. The gametes of the second parent can only be Wb.

The resulting offspring will be:

1/2 wWBb , White wool and brown eyes.
1/2 wWbb , White wool and blue eyes.

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3 years ago

To give birth to her infant, a woman is asked to push with contractions. which pushing technique is the most effective and safes

lesya [120]

While giving birth to her infant during labor, the safest and the most effective pushing technique taught to a woman during contractions is grasping knees, head elevated and breathing out.

During labor, it is very important for the mother to keep breathing. If she holds her breath, it will put pressure on the vena cava and lead to a reduction in the blood return. The head is kept elevated and the back is rounded which aids the abdominal muscles to help the uterus to push the baby out. Grasping the knees also helps in pushing and encouraging the movement of the infant outside the body.

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2 years ago

Why are nitrogen oxides considered harmful to humans?

Stolb23 [73]

Answer:

Nitrogen dioxide is an irritant gas, which at high concentration cause inflammation of the airways. ... Nox gases react to form smog and acid rain as well as being central to the formation of fine particles and ground level ozone, both of which are associated with adverse health effects.

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2 years ago

Read 2 more answers