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2 years ago

What is the specific application of reverse transcriptase in the preparation of cdna?

1 answer:

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The specific application involved in reverse transcriptase preparation of cDNA is a synthesis of DNA to form an RNA-DNA duplex.

What is reverse trancriptase activirt in cDNA?

Recombinant RNA-dependent DNA polymerases known as reverse transcriptases are employed in molecular biology to catalyze the conversion of RNA into DNA during a first-strand cDNA synthesis reaction.
A double helix molecule with an RNA-DNA hybrid is created by this process. When an RNA fragment is complexed with DNA, reverse transcriptases feature an RNase H domain with endoribonuclease activity that specifically breaks down RNA.
The RNA fragment can only be transcribed once thanks to the degradation of RNA in an RNA-DNA complex, which minimizes transcription bias and ensures reaction fidelity.

Reverse transcriptases from either the avian myeloblastosis virus (AMV) or the Moloney murine leukaemia virus (MMLV) are used in the majority of commercial reverse transcription kits.

Compared to AMV, MMLV provides a number of benefits, but its reduced error rate and greater processivity rate stand out.

Hence, the correct answer is a synthesis of DNA to form an RNA-DNA duplex.

Learn more about RNA here,

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Which cell types are not part of the nervous system?.

tresset_1 [31]

Answer:

Neutrophils and Macrophages

Explanation:

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2 years ago

Climate change can have a large impact on coral reefs. How does climate change impact coral?

AysviL [449]

The climate change impacts a coral in many different ways. But the main reason that could happen is if the climate were a coral lives is too cold and it begans to die. Corals have feelings toi, when they get a sense that they are going to die they try to leave but if they can't they will just die.

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3 years ago

, the cortical angiome: an interconnected vascular network with noncolumnar 663 patterns of blood flow. nat. neuroscience 16, 88

kolezko [41]

Noncolumnar patterns of blood flow with the interconnected vascular network.

The standard model of cortical columns has network has tightly linked vascular network. It graph was carefully studied and theoretically analyzed. The results concluded that the microvascular that forms the subsurface, has formed a topology by the interconnected loops. And it is consistent with the boundary and the position of columns.

Furthermore, it was calculated that the blood flow patterns in the cortical column network are not related to the column's location. Instead, to limit lateral perfusion venues were penetrated that drained the sourced blood effectively by penetrating the arterioles.

Therefore, this analysis helps in understanding the effect of penetration strokes of vessels and its functional imaging on the viability of the brain.

To know more about arteriole, refer to the below link:

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2 years ago

PLZ ANSWER SOON What did J.J. Thomson’s experiments with cathode-ray tubes imply about the mass of an electron?

vovikov84 [41]

Answer:

Electrons are many thousand times smaller than the nucleus and negatively charged.

Explanation:

Electrons possess negligible mass and carry a negative charge.

If you would like FREE tutoring in chemistry or another subject, check out www.growthinyouth.org.

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3 years ago

Compare carbon cycled in the hydrosphere with carbon cycled in the geosphere

Natali [406]

Answer:

C Volcanic activity releasing carbon into the atmosphere.

Explanation:

The statement that best describes the cycling of carbon between the atmosphere and geosphere among the options is volcanic activity that leads to releasing carbon into the atmosphere.

Volcanic activities has to do with the activity of the geological layer of the earth that leads to the emission of gases, eruption of lava or the combination of the two.

When a volcanic activity leads to the release of carbon related gases such as carbon dioxide into the atmosphere, carbon is being circulated between the geosphere (the earth) and the atmosphere (the air).

Hence, the correct option is C.

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3 years ago