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1 year ago

fret-based sensor for camkii activity (fresca): a useful tool for assessing camkii activity in response to

1 answer:

7 0

fret-based sensor for CAMKII activity (Fresca): a useful tool for assessing camkii activity in response to Ca2+ oscillations in live cells - PMC.

For embryogenesis as well as neuronal, immune, and cardiac signaling, Ca2 oscillations and subsequent Ca2/calmodulin-dependent protein kinase II (CaMKII) activation are necessary. Fertilization causes Ca2 oscillations, but the pattern of CaMKII activity that follows is still mostly unknown. To fill this gap, we started by using the sole CaMKII activation biosensor that is currently available.

Since CaMKII is a component of this sensor, Camui, it only records when this particular CaMKII variation is activated. Additionally, we developed a substrate-based CaMKII activity sensor, FRESCA (FRET-based) to simultaneously detect the activity of all endogenous CaMKII variants. CaMKII activity sensor). To investigate the difference responses of the sensors Camui and FRESCA.

To learn more about Ca2+ oscillations here

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