Answer:
- DNA to be cloned
- mature mammalian mRNA is isolated
- reverse transcriptase, provided with a primer, synthesizes a strand of cDNA without introns
- the mRNA is hydrolyzed by raising the pH
- a primer site is created on the ssDNA
- a complementary DNA strand is synthesized, forming a ds cDNA molecule.
- dsDNA
Explanation:
cDNA is the acronym for complementary DNA. This DNA is a single or double stranded moolecule that is synthesized from a messenger RNA molecule. This molecule is used in studies that aim to clone eukaryotic genes in prokaryotic individuals. The result of this is the expression of a protein from a eukaryotic individual in prokaryotic individuals.
To form a double-stranded cDNA molecule, the following steps would be required:
- DNA to be cloned
- mature mammalian mRNA is isolated
- reverse transcriptase, provided with a primer, synthesizes a strand of cDNA without introns
- the mRNA is hydrolyzed by raising the pH
- a primer site is created on the ssDNA
- a complementary DNA strand is synthesized, forming a ds cDNA molecule.
- dsDNA
Answer:
They are neither plants nor animals. They are organisms that are classified under the Kingdom Protista.
Explanation:
Answer:
2 electrons
Explanation:
According to the octet rule, atoms must bond to each other, sharing electrons among themselves in an attempt to complete their valence shell (last layer of the electrosphere). In other words, an atom becomes stable when it has 8 electrons in its valence shell.
Oxygen atoms have six electrons in their valence shell, so to achieve the stability suggested by the octet rule (eight electrons), these atoms share two electrons, forming one oxygen gas (O₂) molecule.
