Question

Course heroare we doing a serial dilution of e. coli for the phage titer experiment? why or why not?

Answer 1

The method of determining phage attention by dilution and plate with susceptible cells is named titering or the plaque assay. This process defines the number of viable phage particles in a stock suspension.

Dilution factor

First, we need to estimate the dilution factor. Three successive dilutions were made: 1/100 the first, 1/100 the second, and 1/10 the third part. The original stock was therefore diluted by 100,000 spans. One-tenth of one mL of the definitive dilution was plated, and 65 plaques occurred on the plate. Accordingly, there were 650 functional phage particles per mL (650 PFU/mL) in the final dilution. The authentic stock contained therefore 650 PFU/mL X 100,000 = 65,000,000 PFU/mL.

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