Question

If ecori is applied to a large, complex dna sample (such as as a whole genome) the result should be:________

Answer 1

The DNA conformation changes when ecori is applied to a big, complicated DNA sample, changing from a rod-like to a bent shape C.

Describe Eco-RI.

The bacterium Escherichia coli contains the globular type II restriction enzyme known as Eco-RI endonuclease (Eco-RI). Like many other endonucleases found in bacteria and archaea, this one serves as a defense mechanism to keep the cell from being overrun by foreign DNA.

Describe EcoRV-HF.

EcoRV-HF was designed for lower star activity, although it has the same specificity as EcoRV . Cleaves in the tetracycline resistance gene of pBR322 to leave a ligatable blunt end. some combinations of overlapping CpG methylation are impaired.

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