Acid-fast and Gram is used to visualize the structures in a membrane.
It is a differential staining procedure which is used to identify, <em>mycobacterium tuberculosis and M. leprae.</em>
Acid-fast bacteria are considered to be gram-positive in terms of their structure as, they contains peptidoglycan in its cell wall.
They stain poorly with Gram stain procedure, and appears as weak Gram-positive.
Membranes are consisted of lipid bilayer, which is doubled layer of phospholipid, glycolipid and cholesterol molecules, which contains the chains of fatty acids, and
<em>determines weather a membrane is formed into long flat sheets or round vesicles.</em>
To examine the membrane protein structure, Electron microscopy technology is used and it enables the visualization of single protein at nanometers resolution.
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