mbb347 considering the DNA fragments in the ligation reaction list all of the possible ligation products that could occur. Gene segment and PCR of these products will confer kanamycin resistance in transformed bacteria.
Three pathways are thought to be responsible for bacterial resistance to kanamycin. One technique involves a transposon-borne aminoglycoside-modifying enzyme.
Specific rRNA methylation is the second pathway. The cause of kanamycin resistance was modification of the rRNA at position 1405 or 1408. An antibiotic used to treat tuberculosis and severe bacterial infections is kanamycin A, sometimes known as kanamycin.
Antibiotic usage is the primary contributor to antibiotic resistance. While some bacteria die when humans take antibiotics, resistant bacteria can live and even proliferate.
Antibiotic usage increases the prevalence of microorganisms with resistance. Bacteria have a greater probability of developing antibiotic resistance the more frequently we use antibiotics.
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