When every dilution of your sample results in a plate that is too few to count we should filter the original sample and use most probable number method .
Probable number method count and determine the number of bacteria in a culture, plate aliquots of the dilutions onto agar with sterile pipettes, spread with glass sticks, incubate at 37°C and count the number of colonies. It is imperative that you utilize your best culture technique.
Not all bacterial cells produce colonies, as some bacteria tend to clump or aggregate, and some are nonviable. For this reason results are reported as colony forming units (CFU)/ml of bacterial culture. Ideally only plates with 25-250 colonies are used.
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