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Liono4ka [1.6K]
1 year ago
10

How are the dna replication products visualized during the sanger method of dna sequencing?.

Biology
2 answers:
n200080 [17]1 year ago
5 0

The DNA replication products visualized during the sanger method of DNA sequencing are observed in which nucleotides are added.

Sanger sequencing is based on the process of DNA replication. A scientist creates a copy of his DNA strand. Then observe which nucleotides have been added. This way you can see the sequence of nucleotides. A laser excites the fluorescent labels in each band and a computer detects the resulting light.

Sanger sequencing produces extension products of various lengths ending in dideoxynucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer. Selective incorporation of chain-terminating dideoxynucleotides by DNA polymerases during in vitro DNA replication.

Learn more about DNA replication here:-brainly.com/question/21265857

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Burka [1]1 year ago
5 0

This is often done using a denaturing polyacrylamide urea gel, with four reactions each run in one of the four lanes (lanes A, T, G, C). The DNA bands are then visualized by autoradiography or UV light and the DNA sequence can be read directly from the X-ray film or gel image.

In automated Sanger sequencing, a computer sequentially reads each band on a capillary gel and uses fluorescence to determine the identity of each terminal d dNTP. In essence, a laser excites the fluorescent labels in each band and a computer detects the resulting emitted light.

Sanger sequencing produces extension products of variable length that terminate in dideoxy nucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer.

Learn more about  Sanger sequencing here:

brainly.com/question/26101335

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Proteins that are functionally less important for the survival of an organism generally evolve _____ more important proteins.
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Proteins that are functionally less important for the survival of an organism generally evolve faster than more important proteins.

Proteins serve as the building blocks for all of life's essential processes. The proteins evolve along with the genes that create them, adding new functionality or features that may potentially result in the development of new species.

The mutation of amino acid-coding nucleotides and the stabilization of novel variations in the population are the two phases required for protein evolution.

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To learn more about protein and mutation here,

brainly.com/question/16238140

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7 0
1 year ago
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Explanation:

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