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Liono4ka [1.6K]
1 year ago
10

How are the dna replication products visualized during the sanger method of dna sequencing?.

Biology
2 answers:
n200080 [17]1 year ago
5 0

The DNA replication products visualized during the sanger method of DNA sequencing are observed in which nucleotides are added.

Sanger sequencing is based on the process of DNA replication. A scientist creates a copy of his DNA strand. Then observe which nucleotides have been added. This way you can see the sequence of nucleotides. A laser excites the fluorescent labels in each band and a computer detects the resulting light.

Sanger sequencing produces extension products of various lengths ending in dideoxynucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer. Selective incorporation of chain-terminating dideoxynucleotides by DNA polymerases during in vitro DNA replication.

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Burka [1]1 year ago
5 0

This is often done using a denaturing polyacrylamide urea gel, with four reactions each run in one of the four lanes (lanes A, T, G, C). The DNA bands are then visualized by autoradiography or UV light and the DNA sequence can be read directly from the X-ray film or gel image.

In automated Sanger sequencing, a computer sequentially reads each band on a capillary gel and uses fluorescence to determine the identity of each terminal d dNTP. In essence, a laser excites the fluorescent labels in each band and a computer detects the resulting emitted light.

Sanger sequencing produces extension products of variable length that terminate in dideoxy nucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer.

Learn more about  Sanger sequencing here:

brainly.com/question/26101335

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When a cell is infected by a virus, __________ is produced and is secreted from the infected cell to protect nearby uninfected c
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The given blank can be filled with interferons.

Explanation:

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When an employee remembers the first manager who came and spoke to them on their first day of work, the event is most likely to
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Episodic Memory!

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6 0
2 years ago
A small family farm has 230 acres of farmland to grow corn . If each acre yields 63 bushels of corn and each bushel can be sold
SashulF [63]

Answer:

$130,410

Explanation:

According to this question, a small family farm has 230 acres of farmland to grow corn. Each acre yields 63 bushels of corn i.e 1 acre = 63 bushels of corn

Also, each bushel can be sold at $9.00 i.e. 1 bushel = $9.00

If 1 bushel = $9.00

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Since 63 bushels of corn = 1 acre, it means that $567 is made on one acre.

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To learn more about Kenneth Doka here

brainly.com/question/14298713

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8 0
1 year ago
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