Question

tructures used in recombinant DNA technology to carry the DNA from the cells of one species to the cells of another species are Multiple Choice donor DNA. sticky ends. cloning vectors. restriction enzymes.

Answer 1

The process of fusing two or more DNA molecules to produce a hybrid is known as recombinant DNA. Restrictions endonucleases and ligases are two classes of enzymes that enable the technique.

When a restriction endonuclease detects a particular DNA sequence, it makes cuts inside or near that sequence. The recognition sequence of a restriction enzyme will haphazardly appear on every (1–4)n bases along a random DNA chain. The equation states how many fragments ends (measured in moles) are produced when a restriction enzyme cuts DNA.

Moles of DNA ends =2x(grams of DNA)/(number of bp)(660 g/mol / bp).

The equation describes how many ends are produced when circular DNA is digested by a restriction enzyme.

Mole ends=2x(moles DNA)x number of restriction sites.

The following equation can be used to determine how many ends are produced during the digestion of a linear molecule by a restriction endonuclease.

Mole ends=2x(molesDNA)number of restriction sites +[2x(moles of DNA)].

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