The process of fusing two or more DNA molecules to produce a hybrid is known as recombinant DNA. Restrictions endonucleases and ligases are two classes of enzymes that enable the technique.
When a restriction endonuclease detects a particular DNA sequence, it makes cuts inside or near that sequence. The recognition sequence of a restriction enzyme will haphazardly appear on every (1–4)n bases along a random DNA chain. The equation states how many fragments ends (measured in moles) are produced when a restriction enzyme cuts DNA.
Moles of DNA ends =2x(grams of DNA)/(number of bp)(660 g/mol / bp).
The equation describes how many ends are produced when circular DNA is digested by a restriction enzyme.
Mole ends=2x(moles DNA)x number of restriction sites.
The following equation can be used to determine how many ends are produced during the digestion of a linear molecule by a restriction endonuclease.
Mole ends=2x(molesDNA)number of restriction sites +[2x(moles of DNA)].
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