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Elodia [21]
3 years ago
8

Help me please thanks

Biology
2 answers:
Reika [66]3 years ago
6 0
I apologize in advance if this is incorrect but what I was taught is that the correct answer is gather data to design a recovery plan.
jekas [21]3 years ago
5 0
The answer is the first one, gathering data to design a recovery plan. 
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Describe the probable effects on gene expression in the lac operon of each mutation: a. Mutation in the lac operator that delete
Stella [2.4K]

Answer:

a. Reduces represor binding.

b. Constitutive expression of the operon.

c. Stronger promoter if it is more similar to the consensus sequence. Weaker promoter if less similar.

Explanation:

The lac operon is an operon required for lactose transport and metabolism in enteric bacteria such as <em>Escherichia coli</em>. <u>It is regulated by glucose and lactose availability</u> and consists of the following structural genes:

  • Lac z gene: encodes the enzyme β-galactosidase, which catalyzes the hydrolysis reaction of lactose into glucose and galactose.
  • Gene lac y: encodes the protein galactoside permease involved in the transport of lactose into the bacterium.
  • Lac a gene: encodes the enzyme thiogalactoside transferase, which catalyzes the transfer of the acetyl group of acetyl coenzyme A to 6-OH of a thiogalactoside acceptor. This gene is not related to lactose metabolism.
  • Promoter: region of DNA recognized by RNA polymerase for transcription.
  • Operator: region of DNA located between the promoter and the beginning of the structural genes, which is recognized by the repressor protein Lac I.
  • Repressor gene (lac I): encodes the Lac I repressor protein, which recognizes the operator region, where it binds. It prevents the transcription of genes under the control of this promoter but stimulates the binding of RNA polymerase. When the repressor is absent (in the presence of inducer which in this case will be lactose or IPTG), RNA polymerase will begin transcription.

The lac operon is under a type of negative regulation, where genes can always be transcribed, except when the Lac I repressor protein is bound to the operon region, for which it has a high affinity. In this case, the promoter of the lac I gene is constitutive, so the Lac I protein is permanently expressed and remains bound in tetramer form to the operon region, preventing the transcription of structural genes.

Since lactose is the inducer of the operon, it is able to bind to the Lac I repressor protein and generate a conformational change that decreases its affinity for the operon region. Thus, the operon region is left free, <u>RNA polymerase can freely transcribe the structural genes and β-galactosidase can degrade lactose to glucose plus galactose</u>. <u>In the absence of lactose, the Lac I repressor protein maintains its high affinity for the operator region, preventing RNA polymerase from transcribing the structural genes</u>. In this way, the system remains closed with consequent energy savings for the bacterium.

As a final clarification, actually the true inducing molecule of the lac operon is called allolactose, an isomer of lactose obtained by a transglycosylation occasionally carried out by β-galactosidase.

a. When there is a mutation in the lac operator that deletes most of the operator, then the repressor Lac I can no longer bind and RNA polymerase will begin transcription.

b. When there is a mutation in the Lac I gene that eliminates the binding of repressor to operator, then this protein can not produce the repression so there would be a constitutive expression of the operon.

c. Since the promoter us a region of DNA recognized by RNA polymerase for transcription, if there is a mutation here it will generate a stronger promoter in the case that this part is similar to a consensus sequence (the most commonly encountered nucleotides found at a certain location). In the case that it is less similar, then it will generate a weaker promoter.

7 0
3 years ago
A new human species was found on a distant planet, whose genetic inheritance and process of reproduction is identical to that of
Vedmedyk [2.9K]

Answer:

  1. E/e ; T/t  ⇒ Square eyes and long tail (Option D)
  2. 3/16 will have round eyes and long tails (Option C)
  3. 1/4 of the progeny will be heterozygous for both traits (Option A)

Explanation:

<u>Available data</u>:

  • Two diallelic unlinked genes
  • Gene E controls eye shape: Dominant allele E expresses square eyes, and recessive allele e expresses round eyes.
  • Gene T controls the tail size. Dominant allele T expresses long trail, and recessive allele t expresses short tail.

<u>Genotypes                                          Phenotypes</u>

EETT, EeTT, EETt, EeTt                     Square eyes and Long tail

eeTT, eeTt                                          Round eyes and Long tail

EEtt, Eett                                             Square eyes and Short tail

eett                                                      Rounf eyes and short tail.

<em>1. An individual of this new species is heterozygous for gene E and heterozygous for gene T. What is their genotype and phenotype?</em>

The heterozygous individual is E/e ; T/t, expressing square eyes and a long tail.

<em>2. Two individuals, who are both heterozygous for eye shape and tail size, mate. Which of the following is a correct statement about the phenotype ratios expected for their offspring?</em>

Cross: between two heterozygous individuals

Parentals) EeTt    x     EeTt

Gametes) ET, Et, eT, et

                ET, Et, eT, et

Punnett square)  .   ET        Et        eT       et

                  ET     EETT    EETt    EeTT    EeTt

                  Et      EETt     EEtt     EeTt      Eett

                  eT    EeTT    EeTt    eeTT     eeTt

                   et     EeTt      Eett     eeTt      eett

F1) Genotype:

  • 1/16 EETT
  • 2/16 EETt
  • 1/16 EEtt
  • 2/16 EeTT
  • 4/16 EeTt ⇒ 1/4 EeTt
  • 2/16 Eett
  • 1/16 eeTT
  • 2/16 eeTt
  • 1/16 eett

      Phenotype

  • 9/16 E-T-, Square eyes and Long tails
  • 3/16 E-tt, Square eyes and short tails
  • 3/16 eeT-, Round eyes and Long tails
  • 1/16 eett, round eyes and short tail

Phenotypic ratio 9:3:3:1

<em>3. From the mating described in question ABOVE, what proportion of ALL of the offspring in will be heterozygous for both traits?</em>

4/16 = 1/4 = 25% of the progeny are expected to be heterozygous for both traits, EeTt.

7 0
3 years ago
As much as 25 percent of the fruits and vegetables grown in
sveta [45]

Answer:

B. Mitigating problems associated with rising sea

Explanation:

8 0
2 years ago
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indicate the sequence of the template strand if a nontemplate strand has the sequence of 5' ATGGGGCGC3'
pshichka [43]
The complementary pair would be 3' TACCCCGCG 5'

I hope I helped!
8 0
3 years ago
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If a male with hemophilia and an unaffected (non-carrier) female had a child, what is the probability that the child will be an
anyanavicka [17]

there is a zero percent chance

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