STEP ONE: Harvesting Radiant Energy
The first step of photosynthesis occurs in the chloroplasts of plant cells. Light photons are absorbed by a pigment called chlorophyll, which is abundant in the thylakoid membrane of each chloroplast. Chlorophyll appears green to the eye because it does not absorb green waves on the light spectrum. It reflects them instead, so that’s the color you see.
STAGE 2: Converting Radiant Energy
After radiant energy from sunlight is absorbed, the plant converts light energy into a usable form of chemical energy to fuel the plant’s cells
STAGE 3: Storing Radiant Energy
The last stage of the photosynthesis process is known as the Calvin-Benson cycle, in which the plant uses atmospheric carbon dioxide and water from soil to convert ATP and NADPH. The chemical reactions that make up the Calvin-Benson cycle occur in the stroma of the chloroplast.
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Explanation: what are the options?
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The TSA or the tryptic soy agar is formed of casein and soybean meal, this formation helps in the appropriate growth of a huge array of non-fastidious and fastidious microbes. This combination of soy and casein provides organic nitrogen in the form of polypeptides and amino acids, which makes the medium more suitable for growth.
In the given case, if one permits the incubation of the dilution tubes for 24 hours prior to plating them on the TSA agar plates than there is a more probability of the result to get affected. As if unlimited resources are already present in the tubes, it will provide more favorable conditions for the formation of more colonies and thus will influence or change the colony-forming units per milliliter.
Even if the dilution is performed in a hood and in an autoclaved medium then also there will be an increase in the colonies of the microbes as in the time of 24 hours interval more microbes will get differentiated and will increase in number.
