When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
B
Explanation:
food source deals with agriculture aka farming
I need more of a sentance to anwser anything
Answer:d)introns; exons
Explanation:Introns are the non-coding portion of the DNA. They do not code for a gene that can be expressed hence the need for them to be removed from the coding genes(Exons). The exons are then joined together after the introns have been removed to create a sequence of amino acids.
