Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
Four new haploid cells are formed once the sister chromatids within the two daughter cells separate, forming four new haploid gametes.
Answer:
The answer is most likely D.
Hope this helps!
<span>The gene sequence containing an operator, promoter and repressor is known as an operon. These genes are called as structural genes. the operon is regulated by the presence/absence of the inducer such as glucose/lactose. It may also be a constitutive operon, which works all the time.</span>
