The major function of the contractile vacuole of amoeba is osmoregulation. The solute concentration found in the cell of amoeba's cytoplasm is more than the solute concentration in the freshwater that surround the external part of the organism, thus, water enter the cell through osmosis. The contractile vacuole collect the excess water and expel it through an opening in the cell membrane. By doing this, the contractile vacuole maintains the water balance in amoeba. This how the contractile vacuole normally operate.
In a situation where amoeba is placed in seawater, then water from the cell cytoplasm will rush out of amoeba cell, because of the higher salt content of the surrounding medium. The contractile vacuole will respond to the situation by increasing its contraction and pumping water out the cell in an accelerated manner, this will lead to the shrinking of the cell. 
 
        
             
        
        
        
Beause we cut down a lot of trees. Often, so many, that the health of a forest is irreparably damaged. A forest is not just a bunch of trees. It is a whole ecosystem, in which the trees anchor the growth of many other plant and animal species, hold the ground together to prevent erosion, and produce a lot of oxygen to support animal life. If you harvest too many of the trees, the local ecosystem stops being a forest and starts turning into something else 
 
        
                    
             
        
        
        
Cutting down trees in the forest to make furniture because trees produce oxygen
        
                    
             
        
        
        
Answer:
The cytosolic and mitochondrial pools of CoA are  kept separate, and no radioactive CoA from the cytosolic pool enters the mitochondrion.
Explanation:
- Fatty acyl group condensed with CoA in the cytosol are first transferred to carnitine and in this process, CoA is released.
- After this, it is transported into the mitochondrion, where it is again condensed with CoA. 
- In this way, the cytosolic and mitochondrial pools of CoA are kept separate, and due to this reason, no radioactive CoA from the cytosolic pool enters the mitochondrion.
- Therefore, according to the given question, the C14 CoA that is added into the liver homogenate along with palmitate shows cytosolic radioactive fraction but not mitochondrial as in the mitochondria a different CoA joins palmitate and not the one containing C14.