You want about 500 calories a day extra.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
The correct ansqwer is- Vitamin D
Explanation:
Vitamin D is the vitamin that can be synthesized by the human body itself therefore it is not an essential vitamin for human beings. Human skin is the part that helps in making this vitamin in humans.
The natural form of vitamin D is vitamin D3 which is formed by the sunlight exposure on the skin. When we expose our skin in the sunlight the UV radiation penetrated our skin and converts provitamin D3 into pre-vitamin D3 then this previtamin can be isomerized into the natural form of vitamin D Which is vitamin D3. So the right answer is vitamin D.
Reactions that hydrolyze the phosphodiester bonds split the DNA molecule between the phosphate groups and the hydroxyl groups of the two sugar groups.
In DNA there is a covalent bond through a phosphate group that connects the hydroxyl group (OH) at the 5' position of the pentose sugar and the hydroxyl group at the 3' position of the pentose sugar of the next nucleotide. This covalent bond is called a phosphodiester bond because chemically the phosphate group is in the diester form.
In other words, the phosphodiester bond connects the sugar in one nucleotide to the sugar in the next nucleotide, so this bond simultaneously connects the two consecutive nucleotides to form a polynucleotide chain. If there is an enzyme that catalyzes the hydrolysis of covalent bonds that combine nucleotides, what happens is that the phosphodiester link between deoxyribose sugars will break.
Learn more about the phosphodiester bonds at brainly.com/question/23660733
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